Recombinant Human Activin RIIA/ACVR2A Protein (His Tag)

Specifications

Catalog number

PKSH031728-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

ACVR2A and ACVR2B are two activin type II receptors. ACVR2A has been shown to interact with INHBA, SYNJ2BP and ACVR1B. The bovine ACVR2A gene encodes a protein of 513 amino acids which is highly homologous (approximately 98% identity) to the rat, mouse, and human ACVR2A proteins. Inactivation of ACVR2A is a common event in prostate cancer cells suggesting it may play an important role in the development of prostate cancer. The ACVR2A gene is a putative tumor suppressor gene that is frequently mutated in microsatellite-unstable colon cancers (MSI-H colon cancers) . Frameshift mutation of ACVR2A may contribute to MSI-H colon tumorigenesis via disruption of alternate TGF-beta effector pathways.

Synonyms

ACTR-IIA; ACTRII; ACTRIIA; ACVR2; ACVR2A; Activin Receptor Type IIA; Activin Receptor Type-2A

UniProt

P27037

Accession Number

NP_001607.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Pro 134

Applications

Cell culture

Field of Research

Signal Transduction; Cancer; metabolism; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.4-3 µg/mL in the presence of 10 ng/mL recombinant Activin A.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

14.9 kDa

Observed Molecular Weight

35-40 kDa

Species

Human