Recombinant Human METAP1 Protein (Fc Tag)

Specifications

Catalog number

PKSH031734

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Processing of the N-terminal initiator methionine or formylated methionine is an essential cellular process conserved from prokaryotes to eukaryotes. The proteolytic removal of N-terminal methionine from nascent peptides is catalyzed by a family of enzymes known as methionine aminopeptidases (MetAPs) and is essential for cell growth. METAP1 and METAP2 have different substrate specificity due to the differences in both size and shape of the active sites. As a member of the M24 family of metalloproteases, METAP1 plays an important role in G (2) /M phase regulation of the cell cycle and may serve as a promising target for the discovery and development of new anticancer agents.

Synonyms

MAP 1; MAP1A; METAP1; MetAP 1; MetAP1A; Methionine aminopeptidase 1; Peptidase M 1

UniProt

P53582

Accession Number

NP_055958.2

Expression System

HEK293 Cells

Tag

N-hFc

Sequence

Met 1-Phe 272

Field of Research

Signal Transduction; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

57.0 kDa

Observed Molecular Weight

60-65 kDa

Species

Human