Recombinant Human EphB4/HTK Protein

Specifications

Catalog number

PKSH031737

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore; EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation; extracellular matrix remodeling; and invasive potential are important mechanisms of tumor suppression. Therefore; Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers.

Synonyms

HTK; MYK1; TYRO11

UniProt

P54760

Accession Number

NP_004435.3

Expression System

HEK293 Cells

Tag

None

Sequence

Met 1-Ala 539

Applications

ELISA

Field of Research

Signal Transduction; Cardiovascular; Neuroscience; Cancer; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized human EphB4 at 2 μg/ml (100 μl/well) can bind human EphrinB2 with a linear range of 1-125 ng/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

57.7 kDa

Observed Molecular Weight

65-70 kDa

Species

Human