Recombinant Human ACVR2B/ActivinR-IIB Protein (Fc Tag)

Specifications

Catalog number

PKSH031743-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

ACVR2A and ACVR2B are two activin type II receptors. ACVR2B is integral to the activin and myostatin signaling pathway. Ligands such as activin and myostatin bind to ACVR2A and ACVR2B. Myostatin, a negative regulator of skeletal muscle growth, is regarded as a potential therapeutic target and binds to ACVR2B effectively, and to a lesser extent, to ACVR2A. The structure of human ACVR2B kinase domain in complex with adenine establishes the conserved bilobal architecture consistent with all other catalytic kinase domains. Haplotype structure at the ACVR2B and follistatin loci may contribute to interindividual variation in skeletal muscle mass and strength. Defects in ACVR2B are a cause of left-right axis malformations.

Synonyms

ACTR-IIB; ACVR2B; Activin Receptor Type IIB; Activin Receptor Type-2B; BMP Type II Receptor; BMP Type-2 Receptor; BMPR-5; Bone Morphogenetic Protein Receptor Type II; Bone Morphogenetic Protein Receptor Type-2

UniProt

Q13705

Accession Number

NP_001097.2

Expression System

HEK293 Cells

Tag

C-hFc

Sequence

Met 1-Thr 134

Applications

Cell culture; ELISA

Field of Research

Signal Transduction; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

1. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.02-0.1 µg/mL in the presence of 10 ng/mL recombinant Activin A. 2. Immobilized Inhibin Human, Mouse, Rat, Cynomolgus, Rhesus Inhibin beta A/Activin A at 2 μg/ml (100 μl/well) can bind Human ACVR2B hFc, the EC50 of Human ACVR2B hFc is 12-60 ng/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

40.0 kDa

Observed Molecular Weight

60-65 kDa

Species

Human