Recombinant Human ALK-2/ACVR1 Protein (His & Fc Tag)

Specifications

Catalog number

PKSH031744

Size / count

200 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

ALK-2, also termed as ACVR1, was initially identified as an activin type I receptor because of its ability to bind activin in concert with ActRII or ActRIIB. ALK-2 is also identified as a BMP type I receptor. It has been demonstrated that ALK-2 forms complex with either the BMP-2/7-bound BMPR-II or ACVR2A /ACVR2B. ALK-1 and ALK-2 presenting in the yeast Saccharomyces cerevisiae are two haspin homologues. Both ALK-1 and ALK-2 exhibit a weak auto-kinase activity in vitro, and are phosphoproteins in vivo. ALK-1 and ALK-2 levels peak in mitosis and late-S/G2. Control of protein stability plays a major role in ALK-2 regulation. The half-life of ALK-2 is particularly short in G1. Overexpression of ALK-2, but not of ALK-1, causes a mitotic arrest, which is correlated to the kinase activity of the protein. This suggests a role for ALK-2 in the control of mitosis. Endoglin is phosphorylated on cytosolic domain threonine residues by the TGF-beta type I receptors ALK-2 and ALK-5 in prostate cancer cells. Endoglin did not inhibit cell migration in the presence of constitutively active ALK-2. Defects in ALK-2 are a cause of fibrodysplasia ossificans progressiva (FOP) .

Synonyms

ACTR-I; ACVR1; ACVR1A; ACVRLK2; ALK-2; ALK2; Activin Receptor Type I; Activin Receptor Type-1; Activin Receptor-Like Kinase 2; FOP; SKR1; Serine/Threonine-Protein Kinase Receptor R1; TGF-B Superfamily Receptor Type I; TSR-I

UniProt

Q04771

Accession Number

NP_001104537.1

Expression System

HEK293 Cells

Tag

C-His-Fc

Sequence

Met 1-Val 124

Applications

ELISA

Field of Research

Signal Transduction; epigenetics and nuclear signal; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measure by its ability to bind with human BMP2 in a functional ELISA.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

39.6 kDa

Observed Molecular Weight

40-45 kDa

Species

Human