Recombinant Human NGAL/Lipocalin-2 Protein (His Tag)

Specifications

Catalog number

PKSH031746

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Lipocalin-2 (LCN2), also known as neutrophil gelatinase-associated lipocalin (NGAL), is a 25 kDa protein belonging to the lipocalin superfamily. It was initially found in activated neutrophils, however, many other cells, like kidney tubular cells, may produce NGAL in response to various insults. This protein is released from injured tubular cells after various damaging stimuli, is already known by nephrologists as one of the most promising biomarkers of incoming Acute Kidney Injury (AKI) . Recent evidence also suggests its role as a biomarker in a variety of other renal and non-renal conditions. Moreover, recent studies seem to suggest a potential involvement of this factor also in the genesis and progression of chronic kidney diseases. NGAL is the first known mammalian protein which specifically binds organic molecules called siderophores, which are high-affinity iron chelators. NGAL, first known as an antibacterial factor of natural immunity, and an acute phase protein, is currently one of the most interesting and enigmatic proteins involved in the process of tumor development. acting as an intracellular iron carrier and protecting MMP9 from proteolytic degradation, NGAL has a clear pro-tumoral effect. In thyroid carcinomas, NGAL is strongly induced by NF-kB, an important factor involved both in tumor growth and in the link between chronic inflammation and neoplastic development. Thus, Lipocalin-2 (LCN2/NGAL) has been implicated in a variety of processes including cell differentiation, proliferation, survival and morphogenesis.

Synonyms

25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; Lipocalin-2; NGAL; Neutrophil gelatinase-associated lipocalin; Oncogene 24p3; Siderocalin LCN2; p25

UniProt

P80188

Accession Number

NP_005555.2

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Gly 198

Applications

Enzyme

Field of Research

Signal Transduction; Cardiovascular; Cancer; immunology; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to bind Iron (III) dihydroxybenzoic acid [Fe (DHBA) 3]. The binding of Fe (DHBA) 3 results in the quenching of Trp fluorescence in recombinant human Lipocalin-2. Recombinant human Lipocalin-2 can bind > 1. 5 μM of Fe (DHBA) 3.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

22 kDa

Observed Molecular Weight

22 kDa

Species

Human