Recombinant Human G-CSFR/CD114 Protein (His Tag)

Specifications

Catalog number

PKSH031748

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Granulocyte Colony Stimulating Factor Receptor (G-CSFR), also known as CD114, which belongs to the cytokine receptor superfamily, is a cell surface receptor for colony stimulating factor 3 (CSF3) . It is a critical regulator of granulopoiesis. This type I membrane protein has a composite structure consisting of an immunoglobulin (Ig) -like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type III (FNIII) domains in the extracellular region. Mutations in the G-CSF receptor leading to carboxy-terminal truncation transduce hyperproliferative growth responses, and are implicated in the pathological progression of severe congenital neutropenia (SCN) to acute myelogenous leukemia (AML) . Additionally, autocrine/paracrine stimulation of G-CSFR may be important in the biology of solid tumors, including metastasis.

Synonyms

CD114; CSF3R; G-CSF R; GCSFR

UniProt

Q99062

Accession Number

NP_000751.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Pro 621

Applications

Cell culture

Field of Research

Cancer; immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to inhibit the GCSF-induced proliferation of NFS60 mouse myeloid cells. The ED50 for this ettect is typically 50-250 ng/mL in the presence of 0.125ng/mL of recombinant human GCSF.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

68.0 kDa

Observed Molecular Weight

92 kDa

Species

Human