Recombinant Human Kallikrein 13/KLK13 Protein (His Tag)

Specifications

Catalog number

PKSH031770

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tissue kallikrein 13 (hK13) ; also known as KLK-L4 (kallikrein-like gene 4) ; is a member of the human tissue kallikrein family of serine proteases having diverse physiological functions in many tissues. The KLK13 gene resides on chromosome 19q13.3-4 along with other 14 members in a gene cluster and shares a high degree of homology. KLK13 is a trypsin-like; secreted serine protease expressed specifically in the testicular tissue including prostate; salivary gland; breast; and testis. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and may play a role in metastasis. KLK13 may be involved in the pathogenesis and/or progression of breast and ovary cancers; and is regarded as a novel cancer biomarker. In addition; KLK13 interacts and forms complexes with several serum protease inhibitors; such as alpha2-macroglobulin; and its expression is regulated by steroid hormones.

Synonyms

KLK-L4; KLK13; KLKL4; Kallikrein-13; Kallikrein-Like Protein 4

UniProt

A0A1R3UCE9

Accession Number

NP_056411.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Ile 262

Applications

Enzyme

Field of Research

Signal Transduction; Cell biology; Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC (R&D Systems, Catalog # ES011) . The specific activity is > 200 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by Lysyl-Endopeptidase for an activated form)

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

28.4 kDa

Species

Human