Recombinant Human EphB6 Protein

Specifications

Catalog number

PKSH031773

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Ephrins are divided into the ephrin-A (EFNA) class and the ephrin-B (EFNB) class based on their structures and sequence relationships. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB6 is an unusual Eph receptor; lacking catalytic capacity due to alterations in its kinase domain. Interestingly; increased metastatic activity is associated with reduced EphB6 receptor expression in several tumor types; including breast cancer. This emphasizes the potential of EphB6 to act as a suppressor of cancer aggressiveness. EphB6 suppress cancer invasiveness through c-Cbl-dependent signaling; morphologic changes; and cell attachment and indicate that EphB6 may represent a useful prognostic marker and a promising target for therapeutic approaches. EphB6 can both positively and negatively regulate cell adhesion and migration; and suggest that tyrosine phosphorylation of the receptor by an Src family kinase acts as the molecular switch for the functional transition. In addition; Ephrin-B2 may be a physiological ligand for the EphB6 receptor.

CAS Number

9000-83-3

Synonyms

HEP

Accession Number

NP_004436.1

Host

HEK293 Cells

Origin Species

Human

Tag

None

Sequence

Met 1-Ser 579

Applications

ELISA

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized human EphB6 at 10 μg/mL (100 μl/well) can bind biotinylated human EFNB2-Fch with a linear range of 1. 56-50 ng/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Molecular Weight

72 kDa

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.