Recombinant Human ESAM Protein (aa 1-248, His Tag)

Specifications

Catalog number

PKSH031781

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Endothelial cell-selective adhesion molecule (ESAM) is a member of JAM family of immunoglobulin superfamily and consists of one V-type and one C2-type immunoglobulin domain; as well as a hydrophobic signal sequence; a single transmembrane region; and a cytoplasmic domain. It is specifically expressed at endothelial tight junctions and on activated platelets. ESAM at endothelial tight junctions participates in the migration of neutrophils through the vessel wall; possibly by influencing endothelial cell contacts. The adaptor protein membrane-associated guanylate kinase MAGI-1 has been identified as an intracellular binding partner of ESAM. Previous studies have indicated that ESAM regulates angiogenesis in the primary tumor growth and endothelial permeability. It suggest that ESAM has a redundant functional role in physiological angiogenesis but serves a unique and essential role in pathological angiogenic processes such as tumor growth.

Synonyms

W117m

UniProt

Q96AP7

Accession Number

NP_620411.2

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Ala 248

Field of Research

Signal Transduction; Tags & Cell Markers; Cardiovascular; Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

25.4 kDa

Observed Molecular Weight

35-40 kDa

Species

Human