Recombinant Human S100A4 Protein (Fc Tag)

Specifications

Catalog number

PKSH031787

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

S100A4, also known as metastasis-associated protein Mtsl, belongs to the family of small calcium-binding S100 proteins containing two EF-hand calcium-binding motifs. In humans at least 20 S100 family members that are distributed tissue specifically have been identified, and are involved in a number of cellular processes as transducers of calcium signal. S100A4 is a symmetric homodimer, and undergoes a relatively large conformational change upon the typical EF-hand binding calcium, which is necessary for S100A4 to interact with its protein targets and generate biological effects. It can bind the already known targets p53, F-actin, liprin β, myosin heavy chain II, and prevent their phosphorylation and multimerization. It has been demonstrated that S100A4 is directly involved in tumor metastasis including cell motility, invasion, apoptosis, angiogenesis and differentiation, and appears to be a metastasis factor and a molecular marker for clinical prognosis. Multiple alternatively spliced variants encoding the same protein have been identified.

Synonyms

18A2;42A; CAPL; Calvasculin; FSP1; MTS1; Metastasin; P9KA; PEL98; Placental calcium-binding protein; Protein Mts1; Protein S100-A4; S100 calcium-binding protein A4; S100A4

UniProt

P26447

Accession Number

NP_002952.1

Expression System

HEK293 Cells

Tag

N-hFc

Sequence

Met 1-Lys 101

Field of Research

Signal Transduction; Cardiovascular; Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

38.4 kDa

Observed Molecular Weight

40 kDa

Species

Human