Recombinant Human IL18RAP/IL1R7 Protein (His Tag)

Specifications

Catalog number

PKSH031797

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin 18 receptor accessory protein, also known as IL18RAP and CDw218b (cluster of differentiation w218b), is an accessory subunit of the heterodimeric receptor for IL18. This protein enhances the IL18 binding activity of IL18R1 (IL1RRP), a ligand binding subunit of IL18 receptor. The coexpression of IL18R1 and this protein is required for the activation of NF-kappaB and MAPK8 (JNK) in response to IL18. IL18RAP is required for the high affinity binding of interleukin 18 (IL-18) to its receptor complex. IL18RAP together with IL18R1 mediates IL-18-dependent activation of NF-kappa-B and JNK. Two putative isoforms of IL18RAP were detected and the ratios and total levels of these isoforms may contribute to the aetiology of coeliac disease. IL18R1 and IL18RAP polymorphisms have been found associated with diseases such as schizophrenia, HSV1 seropositivity and atopic asthma. Analysis of IL18R1 and IL18RAP SNPs in 5 European prospective cohorts suggests that the variability of these genes are unlikely to contribute to modulate the risk of CVD in European populations.

Abbreviation

IL18RAP/IL1R7

Synonyms

IL1R; ACPL; CD218b; CDw218b; IL-18RAcP; IL18RB; IL-18Rbeta; IL-18R-beta; IL-1R7; IL-1R-7; IL-1RAcPL; IL1R7; IL18RAP; IL-1RcPL; CDw218b; IL18Rβ Accessory protein like; Accessory protein-like; CD218 antigen-like family member B; I18RA; IL 18RAcP; IL 1R accessory protein like; IL 1R7; IL 1RAcPL; IL-18 receptor accessory protein; IL18Rbeta; IL-1R accessory protein-like; Interleukin 18 receptor accessory protein; Interleukin-1 receptor 7; Interleukin-18 receptor accessory protein; Interleukin-18 receptor accessory protein-like; Interleukin-18 receptor beta; Interleukin-18 receptor beta chain

UniProt

O95256

Accession Number

NP_003844.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Gly 357

Applications

ELISA

Field of Research

Cancer; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to bind biotinylated Cynomolgus IL18 in a functional ELISA.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

40 kDa

Observed Molecular Weight

50-55 kDa

Species

Human