Recombinant Human Endoglin/CD105 Protein (Fc Tag)

Specifications

Catalog number

PKSH031832

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Endoglin, also known as CD105, is a type I homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated cytoplasmic tail. Endoglin contains an RGD tripeptide which is a key recognition structure in cellular adhesion, , suggesting a critical role for endoglin in the binding of endothelial cells to integrins and/or other RGD receptors. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages. As an accessory receptor for the TGF-β superfamily ligands, endoglin binds TGF-β1 and TGF-β3 with high affinity not by itself but by associating with TGF-β type II receptor (TβRII) and activates the downstream signal pathways. In addition, in human umbilical vein endothelial cells, ALK-1 is also a receptor kinase for endoglin threonine phosphorylation, and mutations in either of the two genes result in the autosomal-dominant vascular dysplasia, hereditary hemorrhagic telangiectasia (HHT) . Endoglin has been regarded as a powerful biomarker of neovascularization, and is associated with several solid tumor types.

Synonyms

CD105; END; ENG; Endoglin; HHT1; ORW1

UniProt

P17813

Accession Number

NP_001108225.1

Expression System

HEK293 Cells

Tag

C-hFc

Sequence

Met 1-Gly 586

Applications

ELISA

Field of Research

Cardiovascular; Cancer; immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

1. Measured by its ability to bind biotinylated Human TGFRB1 in a functional ELISA. 2. Measured by its ability to bind biotinylated Human ALK1-Fc in functional Elisa.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

87.5 kDa

Observed Molecular Weight

115-120 kDa

Species

Human