Recombinant Human CD31/PECAM1 Protein (His Tag)

Specifications

Catalog number

PKSH031833

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin α vβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.

Synonyms

CD31; EndoCAM; GPIIA; PECA1; PECAM-1; PECAM1 ; Platelet endothelial cell adhesion molecule

UniProt

P16284

Accession Number

EAW94208.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Lys 601

Applications

Cell culture

Field of Research

Cardiovascular, Signal Transduction, Stem Cells, Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8x104 cells/well are added to CD31-His coated plates (5μg/mL, 100 μL/well) in the presence of 10 μg/mL PHA, approximately > 70% will adhere after 30 minutes at 37°C.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

66.0 kDa

Observed Molecular Weight

100-110 kDa

Species

Human