Recombinant Human IL-1 beta/IL1B Protein

Specifications

Catalog number

PKSH031839

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL1 is a pleiotropic cytokine. It is involved in the inflammatory response, cell growth, and tissue repair in the cortex. The IL1 superfamily consists of three members, IL1A (IL1 alpha), IL1B (IL1 beta), and IL1 receptor antagonist (IL1Ra) . In clinical, it has been reported that Interleukin (IL) -1 may influence Th1 / Th2 immune responsiveness and has been implicated in the establishment of successful pregnancy. Proinflammatory interleukin (IL) -1 gene polymorphisms associated with high levels of IL-1beta activity increase the risk for hypochlorhydria and distal gastric carcinoma. IL1B polymorphisms may be involved in susceptibility to SSc. Moreover, the IL2-384-G allele may be a marker for the limited phenotype of systemic sclerosis (SSc) .

Abbreviation

IL-1

Synonyms

IL1F; IL-1F; pro IL1F; IL-1; IL1-BETA; IL1F2; IL1 beta; IL1B; catabolin; IL-1B; pro Catabolin; pro IL1B; pro IL1F2; pro leukocytic endogenous mediator; pro leukocytic pyrogen; pro lymphocyte activating factor; pro mononuclear cell factor; IL-1F2; IL 1B; IL-1 beta

UniProt

P01584

Accession Number

NP_000567.1

Expression System

E.coli

Tag

None

Sequence

Ala 117-Ser 269

Applications

Cell culture; ELISA

Field of Research

Cardiovascular; immunology; metabolism

Endotoxin

Please contact us for more information.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

1. Immobilized recombinant human IL1B at 2 μg/mL (100μl/well) can bind recombinant human IL1R2 with a linear range of 6. 25-400 ng/ml. 2. Measured in a cell proliferation assay using D10.G4. 1 mouse helper T cells. The ED50 for this effect is typically 2-8 pg/mL. 3. Immobilized human IL1B at 10 μg/mL (100 μl/well) can bind human IL1R1, The EC50 of human IL1R1 is 0.48 μg/mL. 4. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.4-2 ng/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

17 kDa

Observed Molecular Weight

17 kDa

Species

Human