Recombinant Human IL1R1/CD121a Protein (Fc Tag)

Specifications

Catalog number

PKSH031849

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin 1 receptor, type I (IL-1R1) also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. IL-1R1/CD121a is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA) . IL-1R1/CD121a is an important mediator involved in many cytokine induced immune and inflammatory responses. This protein has been characterized by pharmacological and molecular techniques in the mouse brain. The spindle-shaped astrocytes enclose the wound, separating the healthy from damaged neural tissue. The shape change and subsequent repair processes are IL-1β activity-dependent, acting through the IL-1 type 1 receptor (IL-1R1), as co-application of the IL-1type 1 receptor antagonist protein (IL-1ra) blocks IL-1β induced effects. In the spleen, a slight increase in IL-1R AcP and IL-1R1 was observed during the first hours following LPS stimulation. In conclusion, IL-1R AcP mRNA is expressed in the brain and in other tissues where IL-1R1/CD121a transcripts are found. However, the regulation of its expression is distinct from IL-1R1/CD121a. The high level of expression and the lack of regulation of IL-1R AcP transcripts in the brain under inflammatory conditions suggest that the protein might be constitutively expressed in excess.

Abbreviation

IL1R1/CD121a

Synonyms

D2S; Interleukin-1 receptor type; IL1RT; IL1-R; IL1R1; CD121A; D2S1473; IL-1R-alpha; IL1R; IL1RA; P80; CD121 antigen-like family member A; IL-1 RI; IL1-R1; IL-1R1; IL-1R-1; IL-1RT1; IL-1RT-1; Interleukin-1 receptor alpha; Interleukin-1 receptor type 1; IL1RT1; IL-1R type I; IL-1RI

UniProt

P14778

Accession Number

NP_000868.1

Expression System

HEK293 Cells

Tag

C-hFc

Sequence

Met 1-Thr 332

Applications

ELISA

Field of Research

Cardiovascular; immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

1. Immobilized human IL1-beta at 20 μg/ml (100 μl/well) can bind human IL1R1 Fc chimera with a linear ranger of 6. 4-160 ng/ml. 2. Immobilized human IL1B-his at 10 μg/mL (100 μl/well) can bind human IL1R1-Fc. The EC50 of human IL1R1-Fc is 0.198 μg/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

63.0 kDa

Observed Molecular Weight

80-90 kDa

Species

Human