Recombinant Human IL1F5/IL36RN Protein

Specifications

Catalog number

PKSH031850-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-1 family member 5 (IL-1F5) ; also known as interleukin 36 receptor antagonist (IL36RA) ; is a member of the interleukin 1 cytokine family. This cytokine was shown to specifically inhibit the activation of NF-kappaB induced by interleukin 1 family; member 6 (IL1F6) . IL-1F5 is a highly and a specific antagonist of the IL-1 receptor-related protein 2-mediated response to interleukin 1 family member 9 (IL1F9) . IL-1F5 could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A) ; beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1) ; which is present in epithelial barriers and takes part in local inflammatory response. It has been proved that IL-1F5 induces IL-4 mRNA and protein expression in glia in vitro and enhances hippocampal expression of IL-4 following intracerebroventricular injection. The inhibitory effect of IL-1F5 on LPS-induced IL-1β is attenuated in cells from IL-4-defective mice. Experiment results suggest that IL-1F5 mediates anti-inflammatory effects through its ability to induce IL-4 production and that this is a consequence of its interaction with the orphan receptor; single Ig IL-1R-related molecule (SIGIRR) /TIR8; as the effects were not observed in SIGIRR− /− mice. In contrast to its effects in brain tissue; IL-1F5 did not attenuate LPS-induced changes; or up-regulated IL-4 in macrophages or dendritic cells; suggesting that the effect is confined to the brain.

Abbreviation

IL1F5; IL36RN

Synonyms

FIL; UNQ; PRO; IL1F; IL1L; IL1HY; IL1RP; PSORS; IL36RN; FIL1; FIL1 (DELTA) ; FIL1D; IL-36Ra; IL1F5; IL1HY1; IL1L1; IL1RP3; IL36RA; PSORP; PSORS14; UNQ1896; PRO4342; FIL1 Delta; IL-1 Delta; IL-1F5; IL-1HY1; IL-1L1; IL-1ra Homolog 1; IL-1-Related Protein 3; IL-1RP3; Interleukin-1 Delta; Interleukin-1 Family Member 5; Interleukin-1 HY1; Interleukin-1 Receptor Antagonist Homolog 1; Interleukin-1-Like Protein 1; Interleukin-36 Receptor Antagonist Protein

UniProt

Q9UBH0

Accession Number

Q9UBH0

Expression System

E.coli

Tag

C-His

Sequence

Met 1-Asp155

Applications

Cell culture

Field of Research

Immunology

Endotoxin

< 0.1 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

50

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

17.8 kDa

Observed Molecular Weight

17 kDa

Species

Human