Recombinant Human IL-1RAcP/IL-1R3 Protein (His Tag)

Specifications

Catalog number

PKSH031857

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-1 receptor accessory protein (IL-1RAcP) also known as Interleukin-1 receptor member 3 (IL-1R3) is a a cytokine receptor which binds interleukin 1. The IL-1 receptor accessory protein (IL1RAP) is a transmembrane protein that interacts with IL-1R and is required for IL-1 signal transduction. Interleukin 1 induces synthesis of acute phase and proinflammatory proteins during infection, tissue damage, or stress, by forming a complex at the cell membrane with an interleukin 1 receptor and an accessory protein. IL-1RAcP/IL-1R3 is a necessary part of the interleukin 1 receptor complex which initiates signalling events that result in the activation of interleukin 1-responsive genes. Alternative splicing of this gene results in two transcript variants encoding two different isoforms, one membrane-bound and one soluble. The ratio of soluble to membrane-bound forms increases during acute-phase induction or stress. IL-1RAcP/IL-1R3 mediates interleukin-1-dependent activation of NF-kappa-B. Isoform 1 is part of the membrane-bound form of the IL-1 receptor. Signaling involves formation of a ternary complex containing IL1R1, TOLLIP, MYD88, and IRAK1 or IRAK2. Isoform 2 modulates the response to interleukins by associating with soluble IL1R1 and enhancing interleukin-binding to the decoy receptor.

Abbreviation

IL-1RAcP/IL-1R3

Synonyms

IL1R; Interleukin-1 Receptor; C3orf; IL-1 Receptor Accessory Protein; IL-1R3; IL-1R-3; IL-1RAcP; Interleukin-1 Receptor 3; Interleukin-1 Receptor Accessory Protein; C3orf13; IL1R3; IL1RAP; FLJ37788; IL 1 receptor accessory protein; IL 1R3; IL 1RAcP; IL 1RAP; Interleukin 1 Accessory Protein; interleukin 1 receptor 3; Interleukin 1 receptor accessory protein

UniProt

Q9NPH3

Accession Number

NP_002173.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Glu 359

Field of Research

Cardiovascular; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

40.7 kDa

Observed Molecular Weight

50-55 kDa

Species

Human