Recombinant Human DUSP3/VHR Protein

Specifications

Catalog number

PKSH031861

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Vaccinia H1-related phosphatase (VHR) is classified as a dual-specificity phosphatase (DUSP) ; and the other name is dual-specificity phosphatase 3 (DUSP3) . DUSPs are a heterogeneous group of protein phosphatases that can dephosphorylate both phosphotyrosine and phosphoserine/phosphothreonine residues within the one substrate. Unlike typical DUSPs; VHR lacks mitogen-activated protein kinase (MAPK) -binding domain; and shows poor activity against MAPKs. VHR often act on bisphosphorylated protein substrates; it displays a strong preference for dephosphorylating phosphotyrosine residues over phosphothreonine residues. VHR has been identified as a novel regulator of extracellular regulated kinases (ERKs) . VHR is responsible for the rapid inactivation of ERK following stimulation and for its repression in quiescent cells. VHR is a negative regulator of the Erk and Jnk pathways in T cells and; therefore; may play a role in aspects of T lymphocyte physiology that depend on these kinases.

Synonyms

DUSP3; Dual specificity protein phosphatase 3; Dual specificity protein phosphatase VHR; VHR; Vaccinia H1-related phosphatase

UniProt

P51452

Accession Number

P51452

Expression System

Baculovirus-Insect Cells

Tag

None

Sequence

Met 1-Pro 185

Applications

Enzyme

Field of Research

Signal Transduction; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by hydrolysis of 250 µM 3-O-methyl fluorescein phosphate (OMFP) . The specific activity is > 1.0 µmol/min/m

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

20.6 kDa

Observed Molecular Weight

21 kDa

Species

Human