Recombinant Human IL1R2/CD121b Protein (His Tag)

Specifications

Catalog number

PKSH031865

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin 1 receptor; type II (IL1R2) also known as CD121b (Cluster of Differentiation 121b) is a cytokine receptor that belongs to the interleukin-1 receptor family. This protein binds interleukin alpha (IL1A) ; interleukin beta (IL1B) ; and interleukin 1 receptor; type I (IL1R1/IL1RA) ; and acts as a decoy receptor that inhibits the activity of its ligands. The pleiotropic cytokine IL1 is produced to regulate development and maintenance of the inflammatory responses; and binds to specific plasma membrane receptors on cells. Two distinct types of IL1 receptors which are able to bind IL1 specifically have been identified; designated as IL1RI (IL1RA) and IL1RII (IL1RB) . IL1R1 contributes to IL-1 signaling; whereas the IL-1R2/CD121b has no signaling property and acts as a decoy for IL-1. IL-1R2/CD121b structurally consisting of a ligand binding portion comprised of three Ig-like domains; a single transmembrane region; and a short cytoplasmic domain; is expressed in a variety of cell types including B lymphocytes; neutrophils; monocytes; large granular leukocytes and endothelial cells. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine.

Abbreviation

IL1R2/CD121b

Synonyms

IL1R; Interleukin-1 receptor type; IL-1R; IL1R2; CD121b; CDw121b; IL-1R-2; IL-1RT-2; IL-1RT2; IL1R2c; IL1RB; CD121 antigen-like family member B; IL-1 type II receptor; IL-1R-beta; Interleukin-1 receptor beta; Interleukin-1 receptor type 2; Interleukin-1 receptor type I; IL-1R type II; IL-1RII

UniProt

P27930

Accession Number

NP_004624.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Glu 343

Applications

Cell culture

Field of Research

Cardiovascular; immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

1. Measured by its ability to inhibit IL1β-dependent proliferation in D10.G4. 1 mouse helper T cells. The ED50 for this effect is typically 5. 5 μg/mL in the presence of 40 pg/mL of rhIL1β. 2. Measured by its ability to inhibit Interferon gamma secretion by human natural killer lymphoma NK-92 cells in the presence of 500pg/mL IL1b. The EC50 for this effect is typically 20-80ng/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

39.3 kDa

Observed Molecular Weight

50-55 kDa

Species

Human