Recombinant Human CD155/PVR Protein (His Tag)

Specifications

Catalog number

PKSH031868-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD155; commonly known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5) ; is a type I transmembrane single-span glycoprotein; and belongs to the nectins and nectin-like (Necl) subfamily. CD155 was originally identified based on its ability to mediate the cell attachment and entry of poliovirus (PV) ; an etiologic agent of the central nervous system disease poliomyelitis. The normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. CD155 may assist in an efficient humoral immune response generated within the intestinal immune system. It has been demonstrated that CD155 can be recognized and bond by DNAM-1 and CD96 which promote the adhension; migration and NK-cell killing; and thus efficiently prime cell-mediated tumor-specific immunity.

Synonyms

CD155; HVED; NECL5; Necl-5; PVS; TAGE4

Accession Number

NP_006496.3

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Asn 343

Applications

ELISA

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized human CD155 at 2 μg/ml (100 μl/well) can bind human DNAM1 with a linear ranger of 1. 28-32 ng/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

36.5 kDa

Observed Molecular Weight

60-65 kDa

Species

Human