Recombinant Human Carbonic Anhydrase 9/CA9 Protein (His Tag)

Specifications

Catalog number

PKSH031871-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3– ) and thus participate in a variety of biological and physical processes. CA IX protein is expressed primarily in carcinoma cells lines, and the expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. It is involved in tumorigenesis through many pathways, such as pH regulation and cell adhesion control. CA IX is used as a marker of tumor hypoxia and as a new therapeutic target for many human carcinomas and cancers.

Synonyms

CAIX; Carbonic Anhydrase IX; MN

UniProt

Q16790

Accession Number

NP_001207.2

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Asp 414

Applications

Enzyme

Field of Research

Signal Transduction; Cardiovascular; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its esterase activity. The specific activity is > 30 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 2. 5 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

42.5 kDa

Observed Molecular Weight

48 kDa

Species

Human