Recombinant Human PS6K/RPS6KB1 Protein (GST Tag)

Specifications

Catalog number

PKSH031881

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

PS6K, also known as RPS6KB1, is a serine/threonine-protein kinase. It belongs to the RSK (ribosomal s6 kinase) family. Members of this family function in signal transduction. PS6K is an isoform of p70 ribosomal S6 kinase (S6K) . S6K can be activated by mitogenic stimuli such as growth factors, insulin and cytokines. It phosphorylates the ribosomal protein S6. PS6K also phosphorylates other proteins such as elF4B, eEF2K and SKAR. It is a crucial effector of mTOR (rapamycin) signaling. PS6K is dissociated from the EIF3 complex and activated upon mitogenic stimulation, phosphorylation by the mammalian target of mTOR complex 1 (mTORC1) . Its active form then phosphorylates and activates several substrates in the preinitiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. PS6K also functions in cell proliferation, cell growth and cell cycle progression.

Synonyms

PS6K; RPS6KB1; S6K; S6K-beta-1; S6K1; STK14A; p70 S6KA; p70 (S6K) -alpha; p70-S6K; p70-alpha

UniProt

P23443

Accession Number

P23443

Expression System

Baculovirus-Insect Cells

Tag

N-GST

Sequence

Met 1-Leu525

Field of Research

Signal Transduction; Cell biology; epigenetics and nuclear signal; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

85.4 kDa

Observed Molecular Weight

96 kDa

Species

Human