Recombinant Human CNDP1 Protein (His Tag)

Specifications

Catalog number

PKSH031891

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CNDP1; also known as carnosine dipeptidase 1; glutamate carboxypeptidase-like protein 2 (CPGL-2) or carnosinase 1 (CN1) ; is a member of the M20 metalloprotease family. The CNDP1 gene contains trinucleotide (CTG) repeat length polymorphism in the coding region; which has been demonstrated to be associated with susceptibility to developing diabetic nephropathy; for carnosine protection against the adverse effects of high glucose levels on renal cells. In humans; CNDP1 is secreted from the liver into the serum. In other mammals; including rodents; CNDP1 is expressed exclusively within the kidney and lacks a signal peptide. CNDP1 protein is a secreted homodimeric dipeptidase that specifically hydrolyzes L-carnosine (β -alanyl-L-histidine) ; and is identified as human carnosinase expressed in the brain. CNDP1 has been associated with diabetic nephropathy in Europeans and European Americans; but not African-Americans. It was identified and confirmed as a risk factor; were cross-sectional and mostly in patients with type 2 diabetes. The polymorphisms of CNDP1 can be excluded as a risk factor for nephropathy in type 1 diabetes. In addition; CNDP1 is also suggested to be implicated in the actions of neuroprotection and neurotransmiting.

Synonyms

Beta-Ala-His Dipeptidase; CN1; CNDP Dipeptidase 1; CNDP1; CPGL2; Carnosine Dipeptidase 1; Glutamate Carboxypeptidase-Like Protein 2; HsT2308; Serum Carnosinase

UniProt

Q96KN2

Accession Number

NP_116038.4

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ser27-His507

Applications

Enzyme

Field of Research

Signal Transduction; Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to cleave carnosine (β­Ala­L­His) in a two­step assay. The specific activity is > 250 pmoles/min/μg.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

55.3 kDa

Observed Molecular Weight

60-65 kDa

Species

Human