Recombinant Human ALK-1/ACVRL1 Protein (Fc Tag)

Specifications

Catalog number

PKSH031899-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Activin A receptor, type II-like 1 (ACVRL1), also known as ALK-1 (activin receptor-like kinase 1), is an endothelial-specific type I receptor of the TGF-beta (transforming growth factor beta) receptor family of ligands. On ligand binding, a heteromeric receptor complex forms consisting of two type II and two type I transmembrane serine/threonine kinases. ACVRL1 protein is expressed in certain blood vessels of kidney, spleen, heart and intestine, serving as an important role during vascular development. Mutations in ACVRL1 gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2 and vascular disease.

Synonyms

ACVRL1; ACVRLK1; ALK-1; ALK1; Activin Receptor-Like Kinase 1; HHT; HHT2; ORW2; SKR3; Serine/Threonine-Protein Kinase Receptor R3; TGF-B Superfamily Receptor Type I; TSR-I

UniProt

P37023

Accession Number

NP_000011.2

Expression System

HEK293 Cells

Tag

C-hFc

Sequence

Met 1-Gln 118

Applications

Cell culture

Field of Research

Signal Transduction; Cardiovascular; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

1. Measured by its ability to bind Human ENG-Fc, latent TGFB1-His, mouse ENG-His in functional Elisa. 2. Measured by its ability to inhibit BMP9 induced alkaline phosphatase production by MC3T3E1 mouse chondrogenic cells. David, L. et al. (2007) Blood 109:1953. The ED50 for this effect is typically 5-15 ng/mL in the presence of 2 ng/mL of recombiant human BMP9.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

37.4 kDa

Observed Molecular Weight

45-50 kDa

Species

Human