Recombinant Human BACE1/ASP2 Protein

Specifications

Catalog number

PKSH031900

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Beta-site APP-cleaving enzyme 1 (BACE1) is an aspartic-acid protease important in the formation of myelin sheaths in peripheral nerve cells. In the brain; This protein is expressed highly in the substantia nigra; locus coruleus and medulla oblongata. Strong BACE1 expression has also been described in pancreatic tissue. BACE1 has a pivotal role in the pathogenesis of Alzheimer's disease. In Alzheimer's disease patients; BACE1 levels were elevated although mRNA levels were not changed. It has been found that BACE1 gene expression is controlled by a TATA-less promoter. The translational repression as a new mechanism controlling its expression. And the low concentrations of Ca (2+) (microM range) significantly increased the proteolytic activity of BACE1. Furthermore; BACE1 protein is ubiquitinated; and the degradation of BACE1 proteins and amyloid precursor protein processing are regulated by the ubiquitin-proteasome pathway. It has also been identified as the rate limiting enzyme for amyloid-beta-peptide (Abeta) production.

Synonyms

ASP2; BACE; HSPC104

UniProt

P56817

Accession Number

NP_036236.1

Expression System

HEK293 Cells

Tag

None

Sequence

Met 1-Thr 457

Applications

Enzyme

Field of Research

Cell biology; Neuroscience

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to cleave a fluorescent peptide substrate Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys (Dpn) -Arg-Arg-NH2 (Catalog# ES004, R&D Systems) . Cleavage of ES004 can be measured using excitation and emission wavelength of 320 and 405 nm, respectively. The specific activity is > 3. 5 pmoles/min/μg.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

49.0 kDa

Observed Molecular Weight

65 kDa

Species

Human