Recombinant Human BLyS/TNFSF13B/BAFF Protein (Fc Tag)

Specifications

Catalog number

PKSH031910-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

B lymphocyte stimulator (BLyS) ; also known as TNFSF13B; CD257 and BAFF; is single-pass type II membrane protein; which belongs to the tumor necrosis factor family. BAFF is abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. BAFF is a cytokine and serves as a ligand for receptors TNFRSF13B (TACI) ; TNFRSF17 (BCMA) ; and TNFRSF13C (BAFFR) . These receptors is a prominent factor in B cell differentiation; homeostasis; and selection. BLyS levels affect survival signals and selective apoptosis of autoantibody-producing B cells. Thus; it acts as a potent B cell activator and has been shown to play an important role in the proliferation and differentiation of B cells. Overexpression of BLyS in mice can lead to clinical and serological features of systemic lupus erythematosus (SLE) and Sjö gren's syndrome (SS) . BLyS as an attractive therapeutic target in human rheumatic diseases. The ability of BLyS to regulate both the size and repertoire of the peripheral B cell compartment raises the possibility that BLyS and antagonists thereof may form the basis of a therapeutic trichotomy. As an agonist; BLyS protein may enhance humoral immunity in congenital or acquired immunodeficiencies such as those resulting from viral infection or cancer therapy.

Synonyms

BAFF; BLYS; CD257; DTL; TALL-1; TALL1; THANK; TNFSF20; ZTNF4

UniProt

Q9Y275

Accession Number

Q9Y275-1

Expression System

HEK293 Cells

Tag

N-hFc

Sequence

Ala 134-Leu 285

Applications

Cell culture; ELISA

Field of Research

Cell Biology, Immunology, Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

1. Immobilized recombinant human BAFF at 1 μg/mL (100 μl/well) can bind human TNFRSF17.The EC50 of human TNFRSF17 is 0.07 μg/mL. 2. Measured in a cell proliferation assay in mouse splenocytes. The ED50 for this effect is typically 0.6-3. 2 ng/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

43.7 kDa

Observed Molecular Weight

48-55 kDa

Species

Human