Recombinant Human ALCAM/CD166 Protein (His Tag)

Specifications

Catalog number

PKSH031919

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Activated leukocyte cell adhesion molecule (ALCAM) /Cluster of differentiation (CD166) is a type I transmembrane cell adhesion molecule belonging to the Ig superfamily and a ligand for CD6 that is expressed on T lymphocytes. The extracellular domain of ALCAM contains five Ig-like domains (three Ig-like C2-type domains and two Ig-like V-type domains), of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation. ALCAM mediates both heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. ALCAM/CD6 interaction plays a role in T cell development and T cell regulation, as well as in the binding of T- and B-cells to activated leukocytes. Recently, homophilic (ALCAM-ALCAM) adhesion was shown to play important roles in tight cell-to-cell interaction and regulation of stem cell differentiation. While expressed in a wide variety of tissues, ALCAM is usually restricted to subsets of cells involved in dynamic growth and/or migration, including neural development, branching organ development, hematopoiesis, immune response and tumor progression. And CD166 is regarded as a potential novel breast cancer indicator and therapeutic target.

Synonyms

ALCAM; Activated leukocyte cell adhesion molecule; CD166; CD166 antigen; MEMD

UniProt

Q13740

Accession Number

NP_001618.2

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Ala 526

Applications

ELISA

Field of Research

Tags & Cell Markers; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized human ALCAM-His at 10 μg/ml (100 μl/well) can bind mouse CD6-Fc, The EC50 of mouse CD6-Fc is 0.1-0.22 μg/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

57.4 kDa

Observed Molecular Weight

60-70 kDa

Species

Human