Recombinant Human VCL/Vinculin Protein (His Tag)

Specifications

Catalog number

PKSH031929

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Vinculin (VCL) is a cytoskeletal protein that is closely related to both cell-matrix interactions and cell-cell junctions. VCL is a membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton. The protein contains an acidic N-terminal domain and a basic C-terminal domain separated by a proline-rich middle segment. This protein has multi-ligand properties and has been found to interact with a number of microfilament associated proteins, such as talin, a-actinin, and paxillin, which reportedly bind to either the head or tail domains of vinculin.

Synonyms

CMD1W; CMH15; HEL114; MV; MVCL; Metavinculin; VCL; Vinculin

UniProt

P18206

Accession Number

P18206-2

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Gln 1066

Field of Research

Signal Transduction, Cardiovascular, Isotype, Loading Controls

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

118 kDa

Observed Molecular Weight

115 kDa

Species

Human