Recombinant Human VEGFR2/Flk-1/KDR Protein (His Tag)

Specifications

Catalog number

PKSH031930-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

VEGFR2, also called as KDR or Flk-1, is identified as the receptor for VEGF and VEGFC and an early marker for endothelial cell progenitors, whose expression is restricted to endothelial cells in vivo. VEGFR2 was shown to be the primary signal transducer for angiogenesis and the development of pathological conditions such as cancer and diabetic retinopathy. It has been shown that VEGFR2 is expressed mainly in the endothelial cells, and the expression is upregulated in the tumor vasculature. Thus the inhibition of VEGFR2 activity and its downstream signaling are important targets for the treatment of diseases involving angiogenesis. VEGFR2 transduces the major signals for angiogenesis via its strong tyrosine kinase activity. However, unlike other representative tyrosine kinase receptors, VEGFR2 does not use the Ras pathway as a major downstream signaling but rather uses the phospholipase C-protein kinase C pathway to signal mitogen-activated protein (MAP) -kinase activation and DNA synthesis. VEGFR2 is a direct and major signal transducer for pathological angiogenesis, including cancer and diabetic retinopathy, in cooperation with many other signaling partners; thus, VEGFR2 and its downstream signaling appear to be critical targets for the suppression of these diseases. VEGF and VEGFR2-mediated survival signaling is critical to endothelial cell survival, maintenance of the vasculature and alveolar structure and regeneration of lung tissue. Reduced VEGF and VEGFR2 expression in emphysematous lungs has been linked to increased endothelial cell death and vascular regression.

Synonyms

CD309; FLK-1; FLK1; Fetal liver kinase 1; Flk-1; KDR; Kinase insert domain receptor; Protein-tyrosine kinase receptor flk-1; VEGFR; VEGFR-2; VEGFR2; Vascular endothelial growth factor receptor 2

Accession Number

NP_002244.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Glu 764

Applications

Cell culture; BLI

Field of Research

Signal Transduction; Microbiology; Cardiovascular; Cancer; metabolism; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

1. Using the Octet RED System, the affinity constant (Kd) of human VEGFR2-his bound to biotinylated human VEGF165 was 5.9nM. 2. Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC) . The ED50 for this effect is typically 10-50μg/mL in the presence of 10 ng/mL recombinant human VEGF165.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

84.6 kDa

Observed Molecular Weight

120-130 kDa

Species

Human