Recombinant Human G-CSF/CSF3 Protein (Fc Tag)

Specifications

Catalog number

PKSH031938-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Granulocyte-colony stimulating factor (G-CSF) is a growth factor and an essential cytokine belonging to the CSF family of hormone-like glycoproteins. It is produced by numerous cell types including immune and endothelial cells. Recent animal studies have also revealed that G-CSF activates multiple signaling pathways, such as Akt and also the Janus family kinase-2 and signal transducer and activation of transcription-3 (Jak2-STAT3) pathway, thereby promoting survival, proliferation, differentiation and mobilisation of haematopoietic stem and progenitor cells. G-CSF is a cytokine that have been demonstrated to improve cardiac function and perfusion in myocardial infarction. And it was initially evaluated as a stem cell mobilizer and erythropoietin as a cytoprotective agent. G-CSF prevents left ventricular remodeling after myocardial infarction by decreasing cardiomyocyte death and by increasing the number of blood vessels, suggesting the importance of direct actions of G-CSF on the myocardium rather than through mobilization and differentiation of stem cells. Accordingly, recombinant human (rh) G-CSF has been extensively used in clinical haematology and oncology to enable bone marrow transplantation or to treat chemotherapy-associated neutropenia. In preclinical study, G-CSF improved cardiac function and perfusion by angiomyogenesis and protection of cardiomyocytes in myocardial infarction.

Synonyms

C17orf33; CSF3; Filgrastim; G-CSF; GCSF; Granulocyte Colony-Stimulating Factor; Lenograstim; Pluripoietin

UniProt

P09919

Accession Number

NP_757373.1

Expression System

HEK293 Cells

Tag

N-hFc

Sequence

Ala 30-Pro 204

Applications

Cell culture

Field of Research

Cancer; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

Measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60.The ED50 for this effect is typically 0.2-0.8 ng/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

45.4 kDa

Observed Molecular Weight

48 kDa

Species

Human