Recombinant Human PBEF/NAMPT Protein (His & GST Tag)

Specifications

Catalog number

PKSH031981

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Nicotinamide phosphoribosyltransferase (NAMPT) ; also known as pre-B-cell colony-enhancing factor 1 (PBEF1) or visfatin; is an enzyme belonging to the family of glycosyltransferases; to be specific; the pentosyltransferases. This enzyme participates in nicotinate and nicotinamide metabolism. This enzyme catalyzes the condensation of nicotinamide with 5- phosphoribosyl-1- pyrophosphate to yield nicotinamide mononucleotide; one step in the biosynthesis of nicotinamide adenine dinucleotide. NAMPT is also considered as an essential enzyme mediating granulocyte colony-stimulating factor (G-CSF) -triggered granulopoiesis in healthy individuals and in individuals with severe congenital neutropenia. Intracellular NAMPT and NAD+ amounts in myeloid cells; as well as plasma NAMPT and NAD+ levels; were increased by G-CSF treatment of both healthy volunteers and individuals with congenital neutropenia.

Synonyms

NAMPT; NAmPRTase; Nampt; Nicotinamide phosphoribosyltransferase; PBEF; PBEF1; Pre-B cell-enhancing factor; Pre-B-cell colony-enhancing factor 1; Visfatin

UniProt

P43490

Accession Number

P43490

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Met 1-His 491

Field of Research

Signal Transduction; Cardiovascular; Neuroscience; Cancer; immunology; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

83.3 kDa

Observed Molecular Weight

75 kDa

Species

Human