Recombinant Human Interleukin-35/IL-35 Protein (Fc Tag)

Specifications

Catalog number

PKSH031988

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

The novel Ebi3-IL-12alpha heterodimeric cytokine has been designated interleukin-35 (IL-35), is a member IL12 family cytokine produced by regulatory T cells (Treg), but not by resting or activated effector T cells (Teff). IL-35 is a heterodimeric protein composed of IL-12&alpha, (P35) and IL-27&beta, chains, which are encoded by two separate genes called IL12A and EBI3 (Epstein-Barr-virus-induced gene 3) respectively. Ectopic expression of IL-35 confers regulatory activity on naive T cells, whereas recombinant IL-35 suppresses T-cell proliferation. IL-35 has biological activity and able to expand CD4+CD25+ Treg cells, suppress the proliferation of CD4+CD25- effector cells and inhibit Th17 cell polarization. IL-35 has been shown to be constitutively expressed by regulatory T (Treg) cells CD4(+)CD25(+)Foxp3(+) and suggested to contribute to their suppressive activity. IL-35 is a crucial mediator which provokes CD4+CD25+ T cell proliferation and IL-10 generation, another well-known anti-inflammatory cytokine, along with TGFbeta cytokine. IL-35 is a cytokine can downregulate Th17 cell development and inhibit autoimmune inflammation. It inhibited the differentiation of Th17 cells in vitro. In vivo, IL-35 effectively attenuated established collagen-induced arthritis in mice, with concomitant suppression of IL-17 production but enhanced IFN-gamma synthesis. Thus, IL-35 is a novel anti-inflammatory cytokine suppressing the immune response through the expansion of regulatory T cells and suppression of Th17 cell development.

CAS Number

9000-83-3

Abbreviation

Interleukin-35/IL-35

Synonyms

CLMF, IL-12A, IL-35, Interleukin-35, NFSK, NKSF1, P35

UniProt

Q14213;P29459

Accession Number

Q14213&P29459

Host

HEK293 Cells

Origin Species

Human

Tag

C-hFc

Sequence

Met 1-Lys 229

Applications

ELISA

Field of Research

Cancer;immunology

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to bind biotinylated human IL6RB-Fch in a functional ELISA.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.