Recombinant Human LIF Protein (His Tag)

Specifications

Catalog number

PKSH031991

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Leukemia inhibitory factor (LIF) is a pleiotropic glycoprotein belonging to the IL-6 family of cytokines. It’ s involved in growth promotion and cell differentiation of different types of target cells; influence on bone metabolism; cachexia; neural development; embryogenesis and inflammation. LIF has potent proinflammatory property; being the inducer of the acute phase protein synthesis and affecting the cell recruitment into the area of damage or inflammation. LIF is also one of the cytokines that are capable to regulate the differentiation of embryonic stem cells; hematopoietic and neuronal cells. LIF binds to the specific LIF receptor (LIFR-α ) which forms a heterodimer with a specific subunit common to all members of that family of receptors; the GP130 signal transducing subunit. This leads to activation of the JAK/STAT and MAPK cascades. Due to its polyfunctional activities; LIF is involved in the pathogenic events and development of many diseases of various origin.

Synonyms

CDF; D Factor; DIA; Differentiation-Stimulating Factor; Emfilermin; HILDA; LIF; Leukemia Inhibitory Factor; MLPLI; Melanoma-Derived LPL Inhibitor

UniProt

P15018

Accession Number

P15018

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Phe202

Applications

Cell culture

Field of Research

Cell biology; Neuroscience; Cancer; epigenetics and nuclear signal; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.2-1 ng/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

21.2 kDa

Observed Molecular Weight

35-42 kDa

Species

Human