Recombinant Human EGFR/ErbB1 Protein (Fc Tag)

Specifications

Catalog number

PKSH031995-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

As a member of the epidermal growth factor receptor (EGFR) family; EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF; amphiregulin; TGF-α ; betacellulin; etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation; survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization; the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK; PI3K/PKB and STAT) . In addition; EGFR signaling also has been shown to exert action on carcinogenesis and disease progression; and thus EGFR protein is proposed as a target for cancer therapy currently.

Synonyms

ERBB; ERBB1; HER1; NISBD2; PIG61; mENA

UniProt

P00533

Accession Number

NP_005219

Expression System

HEK293 Cells

Tag

C-hFc

Sequence

Met 1-Gly 645

Applications

ELISA

Field of Research

Signal Transduction; Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized recombinant human EGF at 10 μg/ml (100 μl/well) can bind human EGFR with a linear range of 0.64-400 ng/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

95.0 kDa

Observed Molecular Weight

130-140 kDa

Species

Human