Recombinant Human HER2/ErbB2 Protein (His Tag)

Specifications

Catalog number

PKSH031997-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.

Synonyms

CD340; ENV; ENVW; ERBB2; ERVWE1; HER-2; HER-2/neu; HER2; HERV-7q; HERV-W-ENV; HERV7Q; HERVW; HERVWENV; MLN 1; MLN19; Metastatic lymph node gene 19 protein; NGL; Proto-oncogene Neu; Receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2

UniProt

P04626

Accession Number

NP_004439.2

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Thr 652

Applications

ELISA

Field of Research

Signal Transduction; Tags & Cell Markers; Cancer; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized human Erbb2 at 2. 5 μg/ml (100 μl/well) can bind Herceptin with a linear ranger of 1. 28-32 ng/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

71.0 kDa

Observed Molecular Weight

100-110 kDa

Species

Human