Recombinant Human BLK Protein (His Tag)

Specifications

Catalog number

PKSH032011-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tyrosine-Protein Kinase Blk (BLK) contains one protein kinase domain, one SH2 domain and one SH3 domain. BLK is a non-receptor tyrosine kinase, which is involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Signaling through BLK plays an important role in transmitting signals through surface immunoglobulines and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor. Defects in BLK are a cause of maturity-onset diabetes of the young type 11 (MODY11) .

Abbreviation

BLK

Synonyms

B Lymphocyte Kinase; BLK; MODY11; Tyrosine-Protein Kinase Blk; p55-Blk

Accession Number

P51451

Expression System

E.coli

Tag

C-His

Sequence

Gly2-Pro505

Field of Research

Cell biology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

58.7 kDa

Observed Molecular Weight

50-65 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 500mM NaCl, 1mM DTT, pH 7.4.

Species

Human