Recombinant Human HPD/4HPPD Protein (His Tag)

Specifications

Catalog number

PKSH032028-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

4-Hydroxyphenylpyruvate Dioxygenase (4HPPD) belongs to the 4HPPD family. 4HPPD is a key enzyme in the degradation of tyrosine, which catalyzes the second reaction in the catabolism of tyrosine the conversation of 4-hydroxyphenylpyruvate to homogentisate. 4HPPD exists in homodimer forms, which uses zinc as a cofactor to catalyze the third step in the conversion of L-phenylalanine to fumarate and acetoacetic acid. When the active 4HPPD enzyme concentration is low in the human body, it results in high levels of tyrosine concentration in the blood, which can cause mild mental retardation at birth, and degradation in vision as a patient grows older.

Abbreviation

HPD;4HPPD

Synonyms

4-Hydroxyphenylpyruvate Dioxygenase;4-Hydroxyphenylpyruvic Acid Oxidase;4HPPD; HPD; HPPDase; PPD

UniProt

P32754

Accession Number

P32754

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Met393

Field of Research

Signal Transduction; Cell biology; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

47.1 kDa

Observed Molecular Weight

40-50 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 50mM NaCl, 1mM DTT, 20% Glycerol, pH 8.0.

Species

Human