Recombinant Human MGMT Protein (His Tag)

Specifications

Catalog number

PKSH032031-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

MGMT belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. MGMT involved in the cellular defense against the biological effects of O6-methylguanine in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. MGMT catalyzes the chemical reaction: DNA (containing 6-O-methylguanine) and proteinL-cysteine into DNA (without 6-O-methylguanine) and protein S-methyl-L-cysteine.

Abbreviation

MGMT

Synonyms

6-O-methylguanine-DNAmethyltransferase; MGMT; Methylated-DNA--protein-cysteine methyltransferase; O-6-methylguanine-DNA-alkyltransferase

UniProt

P16455

Accession Number

P16455

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Asn207

Field of Research

Epigenetics and nuclear signal

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

23.8 kDa

Observed Molecular Weight

25 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl,1mM DTT,1mM EDTA,500mM NaCl,0.1%Trition X-100, pH 8.0.

Species

Human