Recombinant Human ACADM/MCAD Protein (His Tag)

Specifications

Catalog number

PKSH032032-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Medium-Chain Specific Acyl-CoA Dehydrogenase (ACADM) is a mitochondrial fatty acid beta-oxidation that belongs to the acyl-CoA dehydrogenase family. ACADM is a homotetramer enzyme that catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. ACADM is specific for acyl chain lengths of 4 to 16. It is essential for converting these particular fatty acids to energy, especially during fasting periods. Defects in ACADM cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death.

Abbreviation

ACADM; MCAD

Synonyms

ACADM; MCAD; Medium-Chain Specific Acyl-CoA Dehydrogenase Mitochondrial

UniProt

P11310

Accession Number

P11310

Expression System

E.coli

Tag

N-His

Sequence

Lys26-Asn421

Field of Research

Signal Transduction; Cardiovascular; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

45.9 kDa

Observed Molecular Weight

42 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Acetate, 10% Trehalose, 0.05% Tween 80, pH 5.0.

Species

Human