Recombinant Human LYPLA1 Protein (His Tag)

Specifications

Catalog number

PKSH032043-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Acyl-Protein Thioesterase 1 (APT-1) is lysophospholipase that belongs to the AB hydrolase 2 family. Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. APT-1 performs on biological membranes to regulate the multifunctional lysophospholipids. It hydrolyzes lysophosphatidylcholine in both monomeric and micellar forms. It hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS; in addition, it also has depalmitoylating activity and low lysophospholipase activity.

Abbreviation

LYPLA1

Synonyms

APT-1; APT1; Acyl-Protein Thioesterase 1; LPL-I; LPL1; LYPLA1; LysoPLA I; Lysophospholipase 1; Lysophospholipase I; hAPT1

UniProt

O75608

Accession Number

O75608

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Asp230

Field of Research

Signal Transduction; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

26.8 kDa

Observed Molecular Weight

25 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 10% Glycerol, pH 8.0.

Species

Human