Recombinant Human ALDH1A1/ALDC Protein (His Tag)

Specifications

Catalog number

PKSH032052-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Aldehyde Dehydrogenase Family 1 Member A1 (ALDH1A1) is a cytoplasmic enzyme that belongs to the Aldehyde Dehydrogenase family. ALDH1A1 is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties and subcellular localizations. ALDH1A1 is the main cytosolic isoform, which has a lower affinity for aldehydes than the mitochondrial enzyme. ALDH1A1 binds free retinal and cellular retinol-binding protein-bound retinal. It can convert/oxidize retinaldehyde to retinoic acid.

Abbreviation

ALDH1A1; ALDC

Synonyms

ALDC; ALDH-E1; ALDH1; ALDH11; ALDH1A1; ALHDII; Aldehyde Dehydrogenase Cytosolic; Aldehyde Dehydrogenase Family 1 Member A1; HEL-9; HEL-S-53e; HEL12; PUMB1; PUMB10; RALDH 1; RALDH1; RalDH1; Retinal Dehydrogenase 1

UniProt

P00352

Accession Number

P00352

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Ser501

Field of Research

Neuroscience; Cancer; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

57.0 kDa

Observed Molecular Weight

60 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 20% Glycerol, 1mM DTT, pH 8.0.

Species

Human