Recombinant Human AKR1C4 Protein (His Tag)

Specifications

Catalog number

PKSH032056-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Aldo-Keto Reductase 1C4/AKR1C4 is a member of the aldo/keto reductase family that consists of more than 40 known enzymes and proteins. AKR1C4 has highly expressed in Liver. It can catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. AKR1C4 catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-α-Androstan-3-α,17-β-diol (3-α-diol) . In addition, AKR1C4 also has some 20-α-Hydroxysteroid Dehydrogenase activity.

Abbreviation

AKR1C4

Synonyms

3-Alpha-HSD1;3-Alpha-Hydroxysteroid Dehydrogenase Type I; AKR1C4; Aldo-Keto Reductase Family 1 Member C4; CDR; CHDR; Chlordecone Reductase; DD-4; DD4; Dihydrodiol Dehydrogenase 4; HAKRA

UniProt

P17516

Accession Number

P17516

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Tyr323

Field of Research

Signal Transduction; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

39.3 kDa

Observed Molecular Weight

35-40 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.

Species

Human