Recombinant Human Alkaline Phosphatase/ALPL Protein (His Tag)

Specifications

Catalog number

PKSH032057-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) is a cell membrane protein which belongs to the alkaline phosphatase family. There are at least four distinct but related alkaline phosphatases in humans: intestinal AP (IAP), placental AP (PLAP), germ cell AP (GCAP) and their genes are clustered on chromosome 2, tissue-nonspecific isozyme (TNAP) which gene is located on chromosome 1. Alkaline phosphatases (APs) are dimeric enzymes, it catalyze the hydrolysis of phosphomonoesters with release of inorganic phosphate. The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. This isozyme may play a role in skeletal mineralization. Mutations in ALPL gene have been linked directly to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones, and this disorder can vary depending on the specific mutation since this determines age of onset and severity of symptoms.

Abbreviation

Alkaline Phosphatase; ALPL

Synonyms

ALPL; AP-TNAP; Alkaline Phosphatase; Alkaline Phosphatase Liver/Bone/Kidney Isozyme; HOPS; TNAP; TNSALP; Tissue-Nonspecific Isozyme

UniProt

P05186

Accession Number

P05186

Expression System

HEK293 Cells

Tag

C-His

Sequence

Leu18-Ser502

Field of Research

Tags & Cell Markers; Cell biology; Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

54.5 kDa

Observed Molecular Weight

65-90 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl,1mM DTT,1mM EDTA,500mM NaCl,0.1%Trition X-100, pH 8.0.

Species

Human