Recombinant Human AGR2 Protein (His Tag)

Specifications

Catalog number

PKSH032079-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Anterior Gradient 2 (AGR2) is an 18-21 kDa member of the PDI family of enzymes. AGR2 is widely expressed in secretory cells, such as small intestine goblet, prostate epithelium, enteroendocrine cells, and multiple carcinoma cell types. AGR2 forms transient disulfide linkages with molecules destined for secretion, possibly aiding protein folding. Expression of AGR2 shows a positive correlation with expression of estrogen receptor in breast carcinoma and a negative correlation with expression of EGF receptor. Mature human AGR2 is 155 amino acids (aa) in length (aa 21 - 175) . Cys81 is presumed to participate in intermolecular bond formation. Over aa 21 - 175, human AGR2 shares 94% aa identity with mouse AGR2.

Abbreviation

AGR2

Synonyms

AG-2; AG2; AGR2; Anterior Gradient Protein 2 Homolog; HPC8; Secreted Cement Gland Protein XAG-2 Homolog; hAG-2

UniProt

O95994

Accession Number

O95994

Expression System

HEK293 Cells

Tag

C-His

Sequence

Arg21-Leu175

Field of Research

Signal Transduction; Cancer; Developmental Biology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

18.9 kDa

Observed Molecular Weight

18 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 200mM NaCl, 10%Glycerol, 0.01%Tween 80, pH8.0.

Species

Human