Recombinant Human AIFM1 Protein (His Tag)

Specifications

Catalog number

PKSH032089-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Apoptosis-Inducing Factor 1, Mitochondrial (AIFM1) is a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. During apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces parthanatos i.e., caspase-independent fragmentation of chromosomal DNA. AIFM1 interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. It binds to DNA in a sequence-independent manner and plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells.

Abbreviation

AIFM1

Synonyms

AIF; AIFM1; Apoptosis-Inducing Factor 1 Mitochondrial; PDCD8; Programmed Cell Death Protein 8

UniProt

O95831

Accession Number

O95831

Expression System

E.coli

Tag

N-His

Sequence

Glu121-Asp613

Field of Research

Cell biology; Cancer; epigenetics and nuclear signal; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

56.2 kDa

Observed Molecular Weight

68 kDa

Formulation

Supplied as a 0.2 μm filtered solution of PBS, 50% Glycerol, 2mM EDTA, 0.5M Argine, 5% Trehalose, pH 7.4.

Species

Human