Recombinant Human APE1/APE Protein

Specifications

Catalog number

PKSH032090-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Apurinic-Apyrimidinic Endonuclease 1 (APE1) is required for efficient DNA base excision repair. When the DNA glycosylase remove the damaged bases; APE1 cleaves the AP site to allow resynthesis and ligation to complete repair. APE1 stimulates the DNA binding activity of many transcription factors; which participate in cancer promotion and progression. APE1 regulates the redox state of multiple transcription factors; such as c-Jun; c-Fos; NF-kB; p53. APEN is also involved in calcium-dependent down-regulation of PTH expression.

Abbreviation

APE1; APE

Synonyms

AP Endonuclease 1; APE; APE-1REF-1; APE1; APEN; APEX; APEX Nuclease; APEX1; APX; Apurinic-Apyrimidinic Endonuclease 1; DNA- (Apurinic or Apyrimidinic Site) Lyase; HAP1; REF1; Redox Factor-1

UniProt

P27695

Accession Number

AAH02338.1

Expression System

E.coli

Tag

None

Sequence

Pro2-Leu318

Field of Research

Cardiovascular; epigenetics and nuclear signal

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

35.6 kDa

Observed Molecular Weight

40 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 10mM HEPES, 100mM KCl, 50% Glycerol, pH 7.4.

Species

Human