Recombinant Human ATG10 Protein (His&T7 Tag)

Specifications

Catalog number

PKSH032097-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ubiquitin-Like-Conjugating Enzyme ATG10 (ATG10) is a ubiquitous 28kDa member of the ATG10 family protein. ATG10 acts as an E2-like enzyme, catalyzes the transfer of ATG12 to ATG5 during in the initial stages of autophagesome formation. The heterodimer of ATG5 and ATG12 subsequntly associates non-covalently with an ATG16 multimer to generate an antophagosome. ATG10 plays a role in the conjugation of ATG12 to ATG5 by interaction with MAP1LC3A. In addition, ATG10 can diretly interact with ATG5 or ATG7.

Abbreviation

ATG10

Synonyms

APG10-Like; ATG10; Autophagy-Related Protein 10; Ubiquitin-Like-Conjugating Enzyme ATG10

UniProt

Q9H0Y0

Accession Number

Q9H0Y0

Expression System

E.coli

Tag

N-T7 & C-His

Sequence

Met 1-Thr190

Field of Research

Cell biology; Cardiovascular; Neuroscience; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

24.5 kDa

Observed Molecular Weight

30 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 8% Sucrose, 20% Glycerol, 0.02% Tween 80, 3mM TCEP, pH 8.5.

Species

Human