Recombinant Human ATG5 Protein

Specifications

Catalog number

PKSH032101-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

ATG5 is an E2 ubiquitin ligase which is necessary for autophagy. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity, dramatically highly expressed in apoptotic cells. It is activated by ATG7, conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate complex detaches from the membrane immediately before or after autophagosome formation is completed. ATG5 plays an important role in the apoptotic process, possibly within the modified cytoskeleton.

Abbreviation

ATG5

Synonyms

APG5-like; APG5L; ASP; ATG5; Apoptosis-specific protein; Autophagy protein 5

UniProt

Q9H1Y0

Accession Number

Q9H1Y0

Expression System

E.coli

Tag

None

Sequence

Met 1-Asp275

Field of Research

Signal Transduction; Cell biology; Cardiovascular; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

32.4 kDa

Observed Molecular Weight

33 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 0.2M NaCl, 1mM DTT, 40% Glycerol, pH 8.0.

Species

Human