Recombinant Human Azurocidin/CAP37 Protein (His Tag)

Specifications

Catalog number

PKSH032102-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Azurocidin is an Azurophil granule antibiotic protein, with monocyte chemotactic and antibacterial activity. The Azurophil granules, specialized lysosomes of the neutrophil, contain at least 10 proteins implicated in the killing of microorganisms. Azurocidin is a member of the serine protease family that includes Cathepsin G, Neutrophil Elastase (NE), and Proteinase 3 (PR3), however, Azurocidin is not a serine proteinase since the active site serine and histidine residues are replaced. Human Azurocidin together with NE and PR3 are expressed coordinately and are packaged together into azurophil granules during neutrophil differentiation. Azurocidin has been identified as a modulator of endothelial permeability and an important multifunctional inflammatory mediator. Neutrophils arriving first at sites of inflammation release Azurocidin which acts in a paracrine fashion on endothelial cells causing the development of intercellular gaps and allowing leukocyte extravasation. Azurocidin thus be regarded as a reasonable therapeutic target for a variety of inflammatory disease conditions.

Abbreviation

Azurocidin; CAP37

Synonyms

AZAMP; AZU; AZU1; Azurocidin; Cationic Antimicrobial Protein CAP37; HBP; HUMAZUR; Heparin-Binding Protein; NAZC; hHBP

UniProt

P20160

Accession Number

P20160

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ile27-Pro250

Field of Research

Microbiology; Cardiovascular; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

25.2 kDa

Observed Molecular Weight

38 kDa

Species

Human